AIM 3D Cell Culture Chips offer a new perspective in studying angiogenesis by allowing the growth of new vascular sprouts in a 3D matrix from a pre-existing endothelial monolayer. With the transparent laminate at the bottom, the dynamic process of angiogenesis can be monitored in detail from a viewing angle that is perpendicular to the direction of sprouting. The two-media- channel design not only enables the generation of concentration gradient of angiogenic stimuli but also the application of interstitial flow that is very useful in inducing angiogenesis. This protocol covers the techniques to obtain consistent angiogenic sprouting in AIM chips.
Users can submit their protocols to be referenced in this section and given due credit.
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- Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting. Chan JM, Zervantonakis IK, Rimchala T, Polacheck WJ, Whisler J, Kamm RD. PLoS ONE, 2012. 7 (12):e50582
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- In vitro angiogenesis assay for the study of cell-encapsulation therapy. Kim C, Chung S, Yuchun L, Kim M-C, Chan JKY, Asada HH, Kamm RD. Lab Chip, 2012. 12 (16):2942-2950
- Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting. Lim SH, Kim C, Aref AR, Kamm RD, Raghunath M. Integr. Biol., 2013. 5 (12):1474-1484
- *Crumbs proteins regulate layered retinal vascular development required for vision. Son S, Cho M and Lee J. Biochemical and Biophysical Research Communications, Volume 521, Issue 4, 22 January 2020, Pages 939-946.