AIM 3D Cell Culture Chips offer a new perspective in studying angiogenesis by allowing the growth of new vascular sprouts in a 3D matrix from a pre-existing endothelial monolayer. With the transparent laminate at the bottom, the dynamic process of angiogenesis can be monitored in detail from a viewing angle that is perpendicular to the direction of sprouting. The two-media- channel design not only enables the generation of concentration gradient of angiogenic stimuli but also the application of interstitial flow that is very useful in inducing angiogenesis. This protocol covers the techniques to obtain consistent angiogenic sprouting in AIM chips.
Users can submit their protocols to be referenced in this section and given due credit.
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