Adherent Cell Migration
AIM 3D Cell Culture Chips are very useful for the study of 3D cell invasion and migration. The chips are not only suitable for endpoint measurement; it can monitor real time cell migration in response to a chemoattractant gradient, making cell trajectory information more readily available. The stimuli may include chemical entities, proteins, genetic regulators, mechanobiological factors, etc.
Unlike Boyden chambers, cell migration can be monitored in real-time and the experiment can be stopped at the optimal time. Cell migration speeds can be quantified easily in AIM chips by determining the distance traveled by cells over a given duration. Compared to scratch assays, AIM chips provide an unambiguous starting point before the cells start to migrate, which yields more reliable & reproducible data. Furthermore, instead of having cells migrate through foreign materials (in Boyden chamber assays) or on a 2D plastic substrate (in scratch or cell exclusion zone assays), AIM chips allow the cell migration or invasion to take place in a 3D matrix that is most relevant to your studies.
The migration of breast cancer cells, MDA-MB-231 and fibrosarcoma cells, HT1080 are used as illustrative examples in the downloadable protocol below..
Users can submit their protocols to be referenced in this section and given due credit.
- Chung, S., et al., Cell migration into scaffolds under co-culture conditions in a microfluidic platform. Lab on a Chip, 2009. 9(2): p. 269-275.
- Polacheck, W.J., J.L. Charest, and R.D. Kamm, Interstitial flow influences direction of tumor cell migration through competing mechanisms. Proc Natl Acad Sci U S A, 2011. 108(27): p. 11115-20.
- Hang, T.-C., et al., Vascular Endothelial Growth Factor (VEGF) and Platelet (PF-4) Factor 4 Inputs Modulate Human Microvascular Endothelial Signaling in a Three-Dimensional Matrix Migration Context. Molecular & Cellular Proteomics : MCP, 2013. 12(12): p. 3704-3718.
- Shin, Y., et al., Hydrogels: Extracellular Matrix Heterogeneity Regulates Three-Dimensional Morphologies of Breast Adenocarcinoma Cell Invasion (Adv. Healthcare Mater. 6/2013). Advanced Healthcare Materials, 2013. 2(6): p. 920-920.
- Kalchman, J., et al., A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow. Microfluidics and Nanofluidics, 2013. 14(6): p. 969-981.
- Polacheck, W.J., et al., Mechanotransduction of fluid stresses governs 3D cell migration. Proceedings of the National Academy of Sciences, 2014. 111(7): p. 2447-2452.
- Kim, M.-C., et al., Cell Invasion Dynamics into a Three Dimensional Extracellular Matrix Fibre Network. PLoS Comput Biol, 2015. 11(10): p. e1004535.